Fig. 7

NF-κB promotes EZH2 gene transcription to upregulate H3K27me3 expression. A RT–qPCR analysis of NFKB1 transcription levels and B ChIP–qPCR analysis of H3K27me3 enrichment at the NFKB1 gene promoter region in THP-1 macrophages treated with PYO and/or EI1 (1 μM for 24 h). C RT–qPCR analysis of EZH2 transcription levels and D ChIP–qPCR analysis of NF-κB enrichment at the EZH2 gene promoter region in THP-1 macrophages treated with PYO and/or Curcumin (10 μM for 12 h). E Western blot analysis of H3K27me3 and EZH2 expression in THP-1 macrophages treated with PYO and/or Curcumin. F Densitometric analysis of H3K27me3 and EZH2 levels normalized to the DMSO group. Data are presented as mean ± SD, n.s. not significant, *P < 0.05, **P < 0.01, ***P < 0.001, n = 3