Fig. 2

PYO upregulates H3K27me3 levels through EZH2. Western blot analysis of A H3K27me3, C EZH2, and E JMJD3 expression in THP-1 macrophages treated with 50 μM PYO for 6 h, with DMSO as control. Densitometric analysis showing H3K27me3 (B), EZH2 (D), and JMJD3 levels (F), normalized to the DMSO group. Western blot analysis of H3K27me3 in THP-1 macrophages treated with G PYO and/or CPI-169 (1 nM for 24 h), and with (I) PYO and/or EI1 (1 μM for 24 h). Densitometric analysis corresponding to panels (G) and (I) are shown in (H) and (J), respectively, displaying the relative H3K27me3 levels normalized to the DMSO group. Data are presented as mean ± SD, **P < 0.01, ***P < 0.001, n = 3