Fig. 9

ALG3 activated the TNF signaling pathway to promote bladder cancer cell behaviors. A Vocanol plot of differentially expressed genes after ALG3 overexpression in UMUC3 cells, obtained from RNA-seq analysis. B, C GO enrichment analysis (B) and Reactome analysis (C) of ALG3-related DEGs to identify altered biological processes. D KEGG enrichment analysis of signaling pathways altered by ALG3-related DEGs. E Western blot analysis of key factors (TNF-α, TNFR1, TRADD, MMP3 and MMP9) in the TNF signaling pathway after transfection with ALG3 cDNA. **P < 0.01, ***P < 0.001