Fig. 7

ALG3 promoted cell proliferation, migration, and invasion of bladder cancer cells. A, B Quantitative real-time PCR analysis of ALG3 expression in T24 and UMUC3 cells after transfection with ALG3 cDNA (A) or ALG3 siRNA (B). C, D Western blot analysis of ALG3 expression in T24 and UMUC3 cells after transfection with ALG3 cDNA (C) or ALG3 siRNA (D) for 48 h. E, F CCK8 assays assessing changes in cell proliferation ability after treatment with ALG3 cDNA (E) or ALG3 siRNA (F). G, H Colony formation assays evaluating cell proliferation ability after transfection with ALG3 cDNA (G) or ALG3 siRNA (H). I, J Transwell assays measuring cell migration and invasion abilities after treatment with ALG3 cDNA (I) or ALG3 siRNA (J). **P < 0.01, ***P < 0.001