Fig. 5

Microarray Analysis and Western Blot Validation of OPN3-Regulated Genes and Pathways in LUAD Cells. a and b The enriched pathways was revealed by the microarray assay, with a heatmap illustrating the relative expression levels of key genes under control and siOPN3 conditions. c and d The expression levels of target protein GPX3 as detected by western blotting in H1299 cells over-expressing OPN3, *P < 0.05; means + SD. e and f The levels of phosphorylated NF-κB (p-NF-κB), total NF-κB p65, CyclinB1, phosphorylated extracellular signal-regulated kinase (p-ERK), total ERK, and OPN3 proteins were measured in NCL-H1299 and H1299 cells over-expressing OPN3, *P < 0.05, ***P < 0.001, ****P < 0.0001; means + SD. g and h The expression levels of target protein GPX3 as detected by western blotting in siOPN3-H1437 cells, ***P < 0.001; means + SD. (i and j) The levels of p-NF-κB, total NF-κB p65, CyclinB1, p-ERK, total ERK, and OPN3 proteins were assessed in NCL-H1437 and siOPN3-H1437 cells using the same method, *P < 0.05, ***P < 0.001; means + SD