Fig. 8

BYSL promotes adipogenic and osteogenic differentiation abilities of radiation-treated hucMSCs via GSK-3β/β-catenin pathway. A Western blot analyses detected the protein expression levels of p-GSK-3β/GSK-3β and β-catenin after BYSL overexpression. B Western blot analyses detected the protein expression levels of p-GSK-3β/GSK-3β and β-catenin after BYSL overexpression and inhibitor of GSK-3β (1-Az) treatment. C Cell proliferation detected using CCK-8 assay. D Cell apoptosis detected using flow cytometric analysis. Adipogenic (E) and osteogenic (F) differentiation abilities of radiation-treated hucMSCs after BYSL upregulation. Scale: 50 μm. *p < 0.05 compared to control group; ^#p < 0.05, ^###p < 0.001 compared to 1 Gy + oe-NC group; ^$p < 0.05, ^$$p < 0.01, ^$$$p < 0.001 compared to 1 Gy + oe-BYSL group