Fig. 6

Knockdown of SERPINE1 improves the effects of quercetin on alleviating ox-LDL-elicited cellular senescence, ROS and lipid accumulation in macrophages. A Representative SA-β-gal staining of ox-LDL-induced RAW264.7 macrophages with transfection of SERPINE1 siRNA (si-SERPINE1) or negative control (si-NC) or/and treatment of quercetin (Que). Scale bar, 50 μm. B Quantification of SA-β-gal-positive mouse RAW264.7 macrophages. C Representative flow cytometry for ROS level in ox-LDL-induced RAW264.7 macrophages with transfection of si-SERPINE1 or si-NC or/and treatment of quercetin. D Quantification of ROS level in the above mouse RAW264.7 macrophages. E Representative Oil red O staining of ox-LDL-induced RAW264.7 macrophages in the context of si-SERPINE1 or si-NC transfection or/and quercetin treatment. Scale bar, 50 μm. F Representative immunofluorescence staining of p16, p21, p53 and SERPINE1 in ox-LDL-induced RAW264.7 macrophages in the context of si-SERPINE1 or si-NC transfection or/and quercetin treatment. Scale bar, 20 μm. G–J Quantification of p16, p21, p53 and SERPINE1 expression. **p < 0.01; ***p < 0.001; ****p < 0.0001; ns, p > 0.05