Fig. 5

Quercetin alleviates cellular senescence, ROS and lipid accumulation and induces autophagy in ox-LDL-treated macrophages. A Representative SA-β-gal staining of mouse RAW264.7 macrophages treated with ox-LDL with or without quercetin (Que). Scale bar, 50 μm. B Quantification of SA-β-gal-positive mouse RAW264.7 macrophages. C Representative flow cytometry for ROS level in mouse RAW264.7 macrophages with treatment of ox-LDL with or without quercetin. D Quantification of ROS level in the above mouse RAW264.7 macrophages. E Representative Oil red O staining of mouse RAW264.7 macrophages in the context of ox-LDL treatment with or without quercetin treatment. Scale bar, 50 μm. F Quantification of lipid droplet area ratio in the above macrophages. G Representative immunofluorescence staining of p16, p21, p53 and SERPINE1 in RAW264.7 macrophages in the context of ox-LDL treatment with or without quercetin treatment. Scale bar, 20 μm. H–K Quantification of p16, p21, p53 and SERPINE1 expression in RAW264.7 macrophages in the context of ox-LDL with or without quercetin. L Representative western blots of SERPINE1 in RAW264.7 macrophages in the context of ox-LDL with or without quercetin. M Quantification of SERPINE1 expression in the above macrophages. N Representative western blots of AMPK, p-AMPK, mTOR, and p-mTOR expression in RAW264.7 macrophages in the context of ox-LDL with or without quercetin. O, P Quantification of p-AMPK/AMPK and p-mTOR/mTOR levels in the above macrophages. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001