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Fig. 3 | European Journal of Medical Research

Fig. 3

From: Quercetin ameliorates ox-LDL-induced cellular senescence of aortic endothelial cells and macrophages by p16/p21, p53/SERPINE1, and AMPK/mTOR pathways

Fig. 3

Quercetin ameliorates ox-LDL-induced cellular senescence of aortic endothelial cells possibly through down-regulating p16 and p21. A, B RT-qPCR analysis of p16 and p21 mRNA expression in HAECs treated with 50 μg/mL ox-LDL combined with 0, 0.5, 1 and 3 μM quercetin (Que). C Representative western blots of p16 and p21 in HAECs in the context of 50 μg/mL ox-LDL treatment combined with 0, 0.5, 1 and 3 μM Que. D, E Quantification of p16 and p21 protein expression in the above HAECs. F, G RT-qPCR analysis of p16 and p21 mRNA expression in ox-LDL-induced HAECs with treatment of Que or transfection of p16 (si-p16) or p21 (si-p21) siRNA or negative control (si-NC). H Representative western blots of p16 and p21 in HAECs in the context of ox-LDL treatment combined with Que treatment or transfection of si-p16, si-p21 or si-NC. I, J Quantification of p16 and p21 protein expression in the above HAECs. K Representative SA-β-gal staining of HAECs with treatment of ox-LDL in combination with Que treatment or transfection of si-p16, si-p21 or si-NC. Scale bar, 50 μm. L Quantification of SA-β-gal-positive HAECs. ***p < 0.001; ****p < 0.0001

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European Journal of Medical Research

ISSN: 2047-783X

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