Fig. 4

The production of NETs was reduced by simvastatin. a The expression of PAD4 in lung tissue sections was detected by immunohistochemistry (scale bar: 50 μm). b Representative immunofluorescence images of CitH3 and DAPI staining (scale bar: 50 μm). c The NET-associated proteins PAD4, NE, Cit-H3 and MPO were determined. The data are presented as the means ± SD. a P < 0.05 vs. the S group; b P < 0.05 vs. the LV group; c P < 0.05 vs. the MS group. N = 12 per group