Fig. 8

MCM8-miRNA regulatory axis in ESCA. a Venn diagram displaying the intersection of 37 miRNAs predicted to interact with MCM8, as identified by the miRWalk and MicroT-CDS databases. b Volcano chart of the differential expression of the 37 intersecting miRNAs in TCGA ESCA dataset showing the seven upregulated (hsa-miR- 105 - 5p, hsa-miR- 335 - 3p, hsa-miR- 940, hsa-miR- 942 - 3p, hsa-miR- 135b- 5p, hsa-miR- 1304 - 3p, hsa-miR- 6783 - 3p) and two downregulated (hsa-miR- 135a- 5p, hsa-miR- 202 - 5p) miRNAs in tumor samples compared with normal controls. c Expression of hsa-miR- 135a- 5p in tumor versus normal samples from TCGA ESCA dataset confirming its downregulation in tumor tissues. d Bioinformatics analysis using RNAhybrid depicting the potential binding site between MCM8 and the downregulated miRNA hsa-miR- 135a- 5p. e Expression of hsa-miR- 202 - 5p corresponding to the downregulation of hsa-miR- 135a- 5p in tumor samples. f RNAhybrid analysis identified the potential binding site between MCM8 and the downregulated miRNA hsa-miR- 202 - 5p