Fig. 6
TEAD4 and DYNLL1 affects tumorigenesis of LA795 cells in mice. Mouse LUAD cells (LA795; 5 × 106 cells per mouse) stably transfected with KD-TEAD4 and OE-DYNLL1 were implanted into BALB/c mice to induce allograft tumors. A Tumor volume measured over a 5-week period. B Representative images of tumors and tumor weight after 5 weeks of growth. C Western blot (WB) analysis for the expression of β-catenin, TEAD4, and DYNLL1 in tumor tissues. D Immunohistochemistry (IHC) for the positive staining of proliferation markers Ki67 and PCNA in tumor tissues. E IHC for the positive staining of Cyclin D1 and p21 levels in tumor tissues. F TUNEL assays to detect cell apoptosis within the tumor tissues. Differences were analyzed by two-way ANOVA (A–F). Each group contained six mice. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001