Fig. 5
DYNLL1 overexpression activates the Wnt/β-catenin pathway and rescues malignant properties of LUAD cells suppressed by TEAD4 knockdown. NCI-1395 and NCI-H441 cells transfected with KD-TEAD4 were additionally administered OE-DYNLL1. A DYNLL1 mRNA expression in cells measured by qPCR analysis. B Colony formation assays to evaluate the colony-forming ability of treated cells. C Immunofluorescence staining for Ki67 to assess cell proliferation. D Flow cytometry analysis of cell apoptosis. E Flow cytometry analysis of cell cycle distribution. F SA-β-Gal (SABG) staining to analyze cell senescence. G Western blot (WB) analysis of Cyclin D1 and p21 protein levels. Each dot corresponds to one independent experiment. Differences were analyzed by two-way ANOVA (A–G). *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001