Fig. 4

TEAD4 mediates transcriptional activation of DYNLL1 in LUAD. A Heatmap of genes correlated with DYNLL1 expression in LUAD, obtained from the UALCAN database. B Intersection of genes related to DYNLL1 expression and potential transcription factors predicted to target DYNLL1, identified using the hTFtarget database. C Correlation between TEAD4 expression and DYNLL1 in LUAD, analyzed using the GEPIA database. D Kaplan–Meier plot analyzing the correlation between TEAD4 expression and patient prognosis in LUAD. E ChIP-seq analysis of TEAD4 binding peaks at the DYNLL1 promoter region. F TEAD4 expression in normal lung epithelial cells (BEAS-2B) and human LUAD cell lines (A549, NCI-H1395, and NCI-H441) measured by qPCR and Western blot (WB). Three KD-TEAD4 plasmids (KD-TEAD4 1#, 2#, 3#) were transfected into NCI-H1395 and NCI-H441 cells using lentiviral vectors. G qPCR analysis of TEAD4 mRNA expression in transfected cells. KD-TEAD4 3# exhibited the strongest knockdown effect and was selected for subsequent experiments. H qPCR analysis of DYNLL1 expression after knockdown of TEAD4 in LUAD cells. I Dual-luciferase reporter assay to evaluate changes in luciferase activity following TEAD4 knockdown in LUAD cells. J ChIP-qPCR analysis of TEAD4 enrichment at the DYNLL1 promoter in LUAD cells. Differences were analyzed by one-way (F) or two-way (G–J) ANOVA. Each dot corresponds to one independent experiment. ***p < 0.001, ****p < 0.0001