Fig. 2

Silencing of DYNLL1 reduces malignant phenotype of LUAD cells and blocks cell cycle progression. Three KD-DYNLL1 plasmids (KD-DYNLL1 1#, 2#, 3#) were transfected into NCI-H1395 and NCI-H441 cells using lentiviral vectors. A DYNLL1 mRNA levels in transfected cells were measured by qPCR, with KD-DYNLL1 2# exhibiting the strongest knockdown effect, selected for subsequent experiments. B Colony formation assay to evaluate the colony-forming ability of KD-DYNLL1-transfected cells. C Immunofluorescence staining for Ki67 to assess cell proliferation potential. D Flow cytometry analysis of cell apoptosis. E Wound healing assays to evaluate cell migration. F Transwell assay to assess cell invasion. G Flow cytometry analysis of cell cycle distribution. H Western blot analysis of protein expression levels of Cyclin D1, p21, and β-catenin in transfected cells. Each dot corresponds to one independent experiment. Differences were analyzed by two-way ANOVA (A–H) *p < 0.05, **p < 0.01, ****p < 0.0001