Fig. 2
From: Inducing mononuclear cells of patients with CADASIL to construct a CSVD disease model
iPSC stemness and trilaminar differentiation assays. A Flow cytometry: SSEA4 (red), OCT4 (yellow), NANOG (blue).The gray lines represent the results of the isotype control and the colored lines represent the results after adding specific marker detection antibodies; the three stemness markers were significantly expressed in the four iPSC cell lines. B Immunofluorescence:OCT4.OCT4 is a stemness marker (red), Hoechst is a nuclear stain (blue), and Merge is an overlay of the OCT4 and Hoechst results. C Tri-germ layer differentiation test. iPSCs cell lines express endoderm markers SOX17 and FOXA2, mesoderm markers Brachyury and α-SMA, and ectoderm markers Pax6 and β-III Tubulin