Fig. 6

The KDM5A/HOXA5 axis regulates osteosarcoma progression in mice by activating the Wnt/β-catenin pathway. We constructed a mouse osteosarcoma model by implanting transduced osteosarcoma cells with lentiviruses carrying shRNA constructs targeting KDM5A (sh-KDM5A), HOXA5 (sh-HOXA5), or a non-targeting control (sh-NC) into immunocompromised mice, selecting subcutaneous tumor implantation. A Mouse osteosarcoma transplanted tumor and growth curve. B Western blot was used to detect the expression of KDM5A, HOXA5, and Wnt/β-catenin path-related proteins in mouse tumor tissues. C IHC measured the level of Ki67 in tumor tissues. *P < 0.05, **P < 0.01, ***P < 0.001