Fig. 5

HOXA5 silence weakened the inhibitory effect of sh-KDM5A on osteosarcoma proliferation and migration and promoted apoptosis via activating Wnt/β-catenin pathway. sh-HOXA5 was transfected into KDM5A knockdown-MNNG and MG63 cells for HOXA5 silence, sh-NC served as the negative control. A qRT-PCR detected HOXA5 level. B Western blot measured the levels of KDM5A, HOXA5, β-catenin, Wnt, GSK3B, p-GSK3B, c-myc proteins. C Cell proliferation was detected by colony formation assay. D Cell migration was detected by wound healing assay. E Cell apoptosis was detected by flow cytometry. * P < 0.05, ** P < 0.01, *** P < 0.001