Fig. 4
From: The KDM5A/HOXA5 axis regulates osteosarcoma progression via activating the Wnt/β-catenin pathway
HOXA5 overexpression inhibited osteosarcoma cell proliferation and migration, and promoted apoptosis via inhibiting Wnt/β-catenin pathway. pcDNA3.1-HOXA5 was transfected into MNNG and MG63 cells for HOXA5 overexpression, pcDNA3.1-NC served as the negative control. A qRT-PCR detected HOXA5 level. B HOXA5 protein levels were detected by western blot. C Western blot measured the levels of β-catenin Wnt, GSK3B, p-GSK3B and c-myc proteins. D Cell proliferation was detected by colony formation assay. E Cell migration was detected by wound healing assay. F Cell apoptosis was detected by flow cytometry. * P < 0.05, ** P < 0.01, *** P < 0.001