Table 1 Primers used in this study
Oligo name | Sequence 5́–3́ | BC | EC | GC% | MW (Da) | *TM (℃) | OD 260 nm | Product length | Oligo ID/metabion international AG |
|---|---|---|---|---|---|---|---|---|---|
Forward primer COL1A2 | TCT CTA CTG GCG AAA CCT GTA | 21 | 197,0 | 47.62 | 6.381 | 59 | 3,5 | 98 | 220823B003H05 1/2 |
Reverse primer COL1A2 | TCC TAG CCA GAC GTG TTT CTT | 21 | 189,0 | 47.62 | 6.363 | 59 | 4,3 | 98 | 220823B003A06 2/2 |