Fig. 5

SAP30 binds to the MT1G promoter region to repress MT1G expression. A Immunofluorescence was used to determine the location of SAP30 in 786-O and CAKI-1 cells. B The MT1G mRNA expression data were obtained from TCGA. C Spearman correlation between MT1G methylation and mRNA expression in the KIRC TCGA dataset. D, E mRNA and protein levels of MT1G were determined in the si-Ctrl, si-SAP30 and HDACi groups. F Immunohistochemical staining for the SAP30, MT1G and p53 proteins in tumour tissues. G, H Correlations between the levels of SAP30 and MT1G and p53 were analysed. I Dual-luciferase reporter assay results showing the activity of the MT1G promoter fragment. J ChIP‒PCR analysis of the binding of SAP30 to the MT1G promoter. K Binding site base pair sequences are indicated with boxes. *p < 0.05, **p < 0.01, ***p < 0.001