Fig. 4

SAP30 knockdown promotes the expression of the MT1G and p53 proteins, while MT1G knockdown reverses the alterations in p53 protein levels, cell proliferation and apoptosis-related phenotypes caused by the knockdown of SAP30. A Venn diagram showing shared genes between the downregulated differentially expressed genes in the KIRC TCGA dataset (|log2FC|> 2; *p value < 0.05) and transcription factors in the ENCODE database. B The top 30 enriched GO terms for 251 genes. C Protein expression of p53 and MT1G in the si-Ctrl, si-SAP30, si-MT1G and si-S + M groups. D–F CCK-8 and colony formation assays were used to analyse the effects of si-Ctrl, si-SAP30, si-MT1G and si-S + M on the proliferation of 786-O and CAKI-1 cells. G, H Flow cytometry was used to detect apoptosis in the si-Ctrl, si-SAP30, si-MT1G and si-S + M groups of 786-O and CAKI-1 cells. *p < 0.05, **p < 0.01, ***p < 0.001