Fig. 2

SAP30 knockdown inhibits the proliferation and promotes the apoptosis of tumour cells. A Protein expression of SAP30 in the si-Ctrl, si-SAP30-1 and si-SAP30-2 groups. B mRNA expression of SAP30 in the si-Ctrl, si-SAP30-1 and si-SAP30-2 groups. C–E CCK-8 and colony formation assays were performed to analyse the effect of si-SAP30 on the proliferation of 786-O, CAKI-1 and OS-RC-2 cells. F, G The numbers of apoptotic cells detected by Hoechst staining in the si-Ctrl and si-SAP30 groups; the arrows indicate apoptotic cells. H–K Flow cytometry was used to detect cell apoptosis and determine the cell cycle phase in the si-Ctrl and si-SAP30 groups of 786-O, CAKI-1 and OS-RC-2 cells. L Expression levels of cell cycle-related proteins in SAP30-knockdown 786-O, CAKI-1 and OS-RC-2 cells. M Expression levels of apoptosis-related proteins in SAP30-knockdown 786-O, CAKI-1 and OS-RC-2 cells. *p < 0.05, **p < 0.01, ***p < 0.001