Fig. 7

Knockdown of H2BC9 affects proliferation, cell cycle, apoptosis and DNA damage in vitro. a,b Knockdown of H2BC9 suppressed the proliferation of Cal-27 and Hep2 cells. c,d Knockdown of H2BC9 induced G2/M arrest of Cal-27 and Hep2 cells. e Knockdown of H2BC9 induced apoptosis of Cal-27 and Hep2 cells. f,g Knockdown of H2BC9 induced DNA damage of Cal-27 and Hep2 cells. Representative images of the comet assay and DNA damage in Cal-27 and Hep2 cells. Scale bars = 100 μm. Unpaired t tests and one-way or two-way analysis of variance were used to determine significance. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001