Fig. 3

In-vitro cellular assay confirming that macrophages in sepsis tend to be M1 polarized. A Flowchart of LPS-stimulated mouse monocyte macrophage leukemia cell line RAW264.7. B CD86 mRNA expression level in RAW264.7 cells. C Expression level of CD206 mRNA in RAW264.7 cells. D The level of IL-1β in RAW264.7 cells. E The level of TNF-α in RAW264.7 cells. F Expression level of IL-4 protein in RAW264.7 cells. G The level of IL-10 in RAW264.7 cells. H Expression level of JAK-STAT signaling pathway proteins in RAW264.7 cells by WB. I Phosphorylation level of STAT1 in RAW264.7 cells. J Phosphorylation level of STAT6 in RAW264.7 cells. Con refers to untreated RAW264.7 cells to be used as a control group, and LPS group refers to RAW264.7 cells treated in 10 ng/mL of LPS for 6 h. All data of three independent trials were expressed as mean ± standard deviation