Fig. 2

Genotyping strategy and identification results for F0 founder animals. a F0 founder animals’ genotyping strategy. b1/b2 b1 Shows the target fragments from F1 to R1 (3.7 kb) and b2 shows the target segment F2 to R2 (3.2 kb) in knockout offspring. c Sequencing results of loxp locus between the 5’arm and 3’arms of the target fragment in knockout offspring; the red bases in c are the loxp sites region